scholarly journals Optimization of DOP-PCR amplification of DNA for high-resolution comparative genomic hybridization analysis

Cytometry ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 317-325 ◽  
Author(s):  
Jacob Larsen ◽  
Anne Marie Ottesen ◽  
Claes Lundsteen ◽  
Henrik Leffers ◽  
J�rgen K. Larsen
Keyword(s):  
High Resolution ◽  
Comparative Genomic Hybridization ◽  
Pcr Amplification ◽  
Hybridization Analysis ◽  
Comparative Genomic ◽  
Comparative Genomic Hybridization Analysis ◽  
Genomic Hybridization

scholarly journals High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1

2001 ◽  
Vol 23 (2) ◽  
pp. 61-64 ◽  
Author(s):  
Jacob Larsen ◽  
Anne Marie Ottesen ◽  
Maria Kirchhoff ◽  
Claes Lundsteen ◽  
Jørgen K. Larsen
Keyword(s):  
Detection Limit ◽  
High Resolution ◽  
B Cell ◽  
Comparative Genomic Hybridization ◽  
Spatial Resolution ◽  
Hybridization Analysis ◽  
Comparative Genomic ◽  
Comparative Genomic Hybridization Analysis ◽  
Genomic Hybridization ◽  
Degenerate Oligonucleotide

We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions were amplified by DOP‐PCR and analysed by means of high resolution comparative genomic hybridization (HR‐CGH) for the evaluation of aberration size detection limit. By means of HR‐CGH, we found the detection limit of DOP‐PCR CGH for deletions to be between 3 Mbp and 7–8 Mbp.

A database system for comparative genomic hybridization analysis

2001 ◽  
Vol 20 (4) ◽  
pp. 75-83 ◽  
Author(s):  
D. Berrar ◽  
W. Dublizky ◽  
S. Solinas-Toldo ◽  
S. Buloshevska ◽  
M. Granzow ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Database System ◽  
Hybridization Analysis ◽  
Comparative Genomic ◽  
Comparative Genomic Hybridization Analysis ◽  
Genomic Hybridization
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